DOI Artikel:
Stanković, Ana: Provisional analysis of DNA contained in skeletal material discovered in Novae in sector IV
DOI Seite / Zitierlink: https://doi.org/10.11588/diglit.41865#0099
Ana Stanković
Warsaw
PROVISIONAL ANALYSIS OF DNA CONTAINED
IN SKELETAL MATERIAŁ DISCOVERED IN NOVAE
IN SECTOR IV
The DNA nucleotide seąuence analysis is considered today as the principal
and commonly used method used to determine the relationship between species
or between individuals belonging to the same species. In archaeology DNA re-
search has been used to search for answers to many different problems chiefly
connected with determining the relationship or identification of organie remains,
human included. The opportunity for carrying out DNA research exists whenev-
er the materiał:
- contains DNA;
- the size of DNA fragments exceeds an average of 200 pairs;
- DNA has not been in contact with coal (product of bonę oxidation);
- a minimum of 50-100 copies of the examined seąuence occurs in a sample
of 0.1 cm3.
In practice, it means that the older the materiał, the lesser the probability of
obtaining results, although the conditions in which the materiał has been pre-
served are ałso of enormous importance. Overall, oxidation conditions do not
favor DNA preservation. To conclude, not every sample will generate a result.
In the above-mentioned research, the most freąuent analysis is that of mito-
chondiral DNA (mtDNA). The strength of this materiał is that:
- many mitochondria are present in a celi (freąuently over 100), hence many
copies of the mitochondrial DNA, while only 2 or 1 сору of the nucleotide se-
ąuence;
- mitochondrial seąuences, especiałly areas HVR1 and HVR2, evolve much
morę ąuickly than the nucleotide seąuences.
Conseąuently, they are much morę varied in a given population or ethnic group.
Simultaneously, uniąue variants of HVR, which сап even be attributed to a sin-
gle family, appear with considerable freąuency [Ivanov, et al. 1996].
In humans mtDNA is inherited from the mother. It is true that during fertili-
zation, the spermatozoon introduces its own specific mitochondria into the egg
celi, but these are immediately degraded together with their DNA. Hence, our
Warsaw
PROVISIONAL ANALYSIS OF DNA CONTAINED
IN SKELETAL MATERIAŁ DISCOVERED IN NOVAE
IN SECTOR IV
The DNA nucleotide seąuence analysis is considered today as the principal
and commonly used method used to determine the relationship between species
or between individuals belonging to the same species. In archaeology DNA re-
search has been used to search for answers to many different problems chiefly
connected with determining the relationship or identification of organie remains,
human included. The opportunity for carrying out DNA research exists whenev-
er the materiał:
- contains DNA;
- the size of DNA fragments exceeds an average of 200 pairs;
- DNA has not been in contact with coal (product of bonę oxidation);
- a minimum of 50-100 copies of the examined seąuence occurs in a sample
of 0.1 cm3.
In practice, it means that the older the materiał, the lesser the probability of
obtaining results, although the conditions in which the materiał has been pre-
served are ałso of enormous importance. Overall, oxidation conditions do not
favor DNA preservation. To conclude, not every sample will generate a result.
In the above-mentioned research, the most freąuent analysis is that of mito-
chondiral DNA (mtDNA). The strength of this materiał is that:
- many mitochondria are present in a celi (freąuently over 100), hence many
copies of the mitochondrial DNA, while only 2 or 1 сору of the nucleotide se-
ąuence;
- mitochondrial seąuences, especiałly areas HVR1 and HVR2, evolve much
morę ąuickly than the nucleotide seąuences.
Conseąuently, they are much morę varied in a given population or ethnic group.
Simultaneously, uniąue variants of HVR, which сап even be attributed to a sin-
gle family, appear with considerable freąuency [Ivanov, et al. 1996].
In humans mtDNA is inherited from the mother. It is true that during fertili-
zation, the spermatozoon introduces its own specific mitochondria into the egg
celi, but these are immediately degraded together with their DNA. Hence, our